human brain vascular pericytes (hpcs Search Results


human brain vascular pericytes (hpcs; passage 1)  (ScienCell)
90
ScienCell human brain vascular pericytes (hpcs; passage 1)
Human Brain Vascular Pericytes (Hpcs; Passage 1), supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+brain+vascular+pericytes+%28hpcs/pm34643436-278-0-10?v=ScienCell
Average 90 stars, based on 1 article reviews
human brain vascular pericytes (hpcs; passage 1) - by Bioz Stars, 2026-07
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fitc-anti-human cd34  (Becton Dickinson)
90
Becton Dickinson fitc-anti-human cd34
Fitc Anti Human Cd34, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc-anti-human cd34 - by Bioz Stars, 2026-07
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mouse stx1a  (OriGene)
90
OriGene mouse stx1a
( A ) Cross-link maps of selected SNARE proteins (dataset 1). Protein domains are depicted based on UniProt database and previous publications [*, from ]. Identified cross-links located in unique and shared peptides of <t>Stx1a</t> and Stx1b are represented as solid and dotted lines, respectively. ( B ) Results of Stxbp1 and control peptide array experiments. Examples of specific, nonspecific, and no binding signals are depicted. Arrowheads indicate antibody positive controls. Quantification and peptide sequences (two independent and two technical replicates) are described in fig. S9B and table S4. ( C ) Comparison of binding regions determined by peptide array (boxes colored in red) and XL-MS (edges). The number of samples in which the cross-links were identified is indicated on the edges (dataset 1). For peptide array assays, full-length proteins are shown as circles, and the protein used to generate peptide sequences in the arrays (Stx1b) is represented as sequence bars. Yellow edges match arrows with the same color in (D). ( D ) Cross-link mapping of Stxbp1 interactions. Stxbp1 (green) and Stx1 (blue) cross-linked sites were mapped onto the Stxbp1-Stx1 complex structure (PDB 3C98; Stx1 in closed conformation). Cross-links are indicated in red (if below DSSO maximal distance restraint) or in magenta (if exceeding DSSO maximal distance restraint). Yellow sticks show the two lysine residues of Stx1 (Lys45 and Lys55) found outside of the binding region with Stxbp1 as defined by the peptide array. Black edge indicates cross-linked site of Snap25.
Mouse Stx1a, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse stx1a - by Bioz Stars, 2026-07
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human cd34þ hpcs isolation human melanoma lines colo829  (ATCC)
95
ATCC human cd34þ hpcs isolation human melanoma lines colo829
( A ) Cross-link maps of selected SNARE proteins (dataset 1). Protein domains are depicted based on UniProt database and previous publications [*, from ]. Identified cross-links located in unique and shared peptides of <t>Stx1a</t> and Stx1b are represented as solid and dotted lines, respectively. ( B ) Results of Stxbp1 and control peptide array experiments. Examples of specific, nonspecific, and no binding signals are depicted. Arrowheads indicate antibody positive controls. Quantification and peptide sequences (two independent and two technical replicates) are described in fig. S9B and table S4. ( C ) Comparison of binding regions determined by peptide array (boxes colored in red) and XL-MS (edges). The number of samples in which the cross-links were identified is indicated on the edges (dataset 1). For peptide array assays, full-length proteins are shown as circles, and the protein used to generate peptide sequences in the arrays (Stx1b) is represented as sequence bars. Yellow edges match arrows with the same color in (D). ( D ) Cross-link mapping of Stxbp1 interactions. Stxbp1 (green) and Stx1 (blue) cross-linked sites were mapped onto the Stxbp1-Stx1 complex structure (PDB 3C98; Stx1 in closed conformation). Cross-links are indicated in red (if below DSSO maximal distance restraint) or in magenta (if exceeding DSSO maximal distance restraint). Yellow sticks show the two lysine residues of Stx1 (Lys45 and Lys55) found outside of the binding region with Stxbp1 as defined by the peptide array. Black edge indicates cross-linked site of Snap25.
Human Cd34þ Hpcs Isolation Human Melanoma Lines Colo829, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd34þ hpcs isolation human melanoma lines colo829 - by Bioz Stars, 2026-07
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purified recombinant gst-tagged rnase l  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology purified recombinant gst-tagged rnase l
( A ) Cross-link maps of selected SNARE proteins (dataset 1). Protein domains are depicted based on UniProt database and previous publications [*, from ]. Identified cross-links located in unique and shared peptides of <t>Stx1a</t> and Stx1b are represented as solid and dotted lines, respectively. ( B ) Results of Stxbp1 and control peptide array experiments. Examples of specific, nonspecific, and no binding signals are depicted. Arrowheads indicate antibody positive controls. Quantification and peptide sequences (two independent and two technical replicates) are described in fig. S9B and table S4. ( C ) Comparison of binding regions determined by peptide array (boxes colored in red) and XL-MS (edges). The number of samples in which the cross-links were identified is indicated on the edges (dataset 1). For peptide array assays, full-length proteins are shown as circles, and the protein used to generate peptide sequences in the arrays (Stx1b) is represented as sequence bars. Yellow edges match arrows with the same color in (D). ( D ) Cross-link mapping of Stxbp1 interactions. Stxbp1 (green) and Stx1 (blue) cross-linked sites were mapped onto the Stxbp1-Stx1 complex structure (PDB 3C98; Stx1 in closed conformation). Cross-links are indicated in red (if below DSSO maximal distance restraint) or in magenta (if exceeding DSSO maximal distance restraint). Yellow sticks show the two lysine residues of Stx1 (Lys45 and Lys55) found outside of the binding region with Stxbp1 as defined by the peptide array. Black edge indicates cross-linked site of Snap25.
Purified Recombinant Gst Tagged Rnase L, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+brain+vascular+pericytes+%28hpcs/pmc11711761-238-3-8?v=MyBiosource+Biotechnology
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purified recombinant gst-tagged rnase l - by Bioz Stars, 2026-07
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activators of rnase l  (Midland Certified Reagent)
90
Midland Certified Reagent activators of rnase l
( A ) Cross-link maps of selected SNARE proteins (dataset 1). Protein domains are depicted based on UniProt database and previous publications [*, from ]. Identified cross-links located in unique and shared peptides of <t>Stx1a</t> and Stx1b are represented as solid and dotted lines, respectively. ( B ) Results of Stxbp1 and control peptide array experiments. Examples of specific, nonspecific, and no binding signals are depicted. Arrowheads indicate antibody positive controls. Quantification and peptide sequences (two independent and two technical replicates) are described in fig. S9B and table S4. ( C ) Comparison of binding regions determined by peptide array (boxes colored in red) and XL-MS (edges). The number of samples in which the cross-links were identified is indicated on the edges (dataset 1). For peptide array assays, full-length proteins are shown as circles, and the protein used to generate peptide sequences in the arrays (Stx1b) is represented as sequence bars. Yellow edges match arrows with the same color in (D). ( D ) Cross-link mapping of Stxbp1 interactions. Stxbp1 (green) and Stx1 (blue) cross-linked sites were mapped onto the Stxbp1-Stx1 complex structure (PDB 3C98; Stx1 in closed conformation). Cross-links are indicated in red (if below DSSO maximal distance restraint) or in magenta (if exceeding DSSO maximal distance restraint). Yellow sticks show the two lysine residues of Stx1 (Lys45 and Lys55) found outside of the binding region with Stxbp1 as defined by the peptide array. Black edge indicates cross-linked site of Snap25.
Activators Of Rnase L, supplied by Midland Certified Reagent, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+brain+vascular+pericytes+%28hpcs/pm09235910-38-2-12?v=Midland+Certified+Reagent
Average 90 stars, based on 1 article reviews
activators of rnase l - by Bioz Stars, 2026-07
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pbabe rnase l  (Thermo Fisher)
99
Thermo Fisher pbabe rnase l
( A ) Cross-link maps of selected SNARE proteins (dataset 1). Protein domains are depicted based on UniProt database and previous publications [*, from ]. Identified cross-links located in unique and shared peptides of <t>Stx1a</t> and Stx1b are represented as solid and dotted lines, respectively. ( B ) Results of Stxbp1 and control peptide array experiments. Examples of specific, nonspecific, and no binding signals are depicted. Arrowheads indicate antibody positive controls. Quantification and peptide sequences (two independent and two technical replicates) are described in fig. S9B and table S4. ( C ) Comparison of binding regions determined by peptide array (boxes colored in red) and XL-MS (edges). The number of samples in which the cross-links were identified is indicated on the edges (dataset 1). For peptide array assays, full-length proteins are shown as circles, and the protein used to generate peptide sequences in the arrays (Stx1b) is represented as sequence bars. Yellow edges match arrows with the same color in (D). ( D ) Cross-link mapping of Stxbp1 interactions. Stxbp1 (green) and Stx1 (blue) cross-linked sites were mapped onto the Stxbp1-Stx1 complex structure (PDB 3C98; Stx1 in closed conformation). Cross-links are indicated in red (if below DSSO maximal distance restraint) or in magenta (if exceeding DSSO maximal distance restraint). Yellow sticks show the two lysine residues of Stx1 (Lys45 and Lys55) found outside of the binding region with Stxbp1 as defined by the peptide array. Black edge indicates cross-linked site of Snap25.
Pbabe Rnase L, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+brain+vascular+pericytes+%28hpcs/pm17130839-136-24-43?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
pbabe rnase l - by Bioz Stars, 2026-07
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human hippocampal progenitor/stem cell line hpc0a07/03c  (Reneuron Inc)
90
Reneuron Inc human hippocampal progenitor/stem cell line hpc0a07/03c
( A ) Cross-link maps of selected SNARE proteins (dataset 1). Protein domains are depicted based on UniProt database and previous publications [*, from ]. Identified cross-links located in unique and shared peptides of <t>Stx1a</t> and Stx1b are represented as solid and dotted lines, respectively. ( B ) Results of Stxbp1 and control peptide array experiments. Examples of specific, nonspecific, and no binding signals are depicted. Arrowheads indicate antibody positive controls. Quantification and peptide sequences (two independent and two technical replicates) are described in fig. S9B and table S4. ( C ) Comparison of binding regions determined by peptide array (boxes colored in red) and XL-MS (edges). The number of samples in which the cross-links were identified is indicated on the edges (dataset 1). For peptide array assays, full-length proteins are shown as circles, and the protein used to generate peptide sequences in the arrays (Stx1b) is represented as sequence bars. Yellow edges match arrows with the same color in (D). ( D ) Cross-link mapping of Stxbp1 interactions. Stxbp1 (green) and Stx1 (blue) cross-linked sites were mapped onto the Stxbp1-Stx1 complex structure (PDB 3C98; Stx1 in closed conformation). Cross-links are indicated in red (if below DSSO maximal distance restraint) or in magenta (if exceeding DSSO maximal distance restraint). Yellow sticks show the two lysine residues of Stx1 (Lys45 and Lys55) found outside of the binding region with Stxbp1 as defined by the peptide array. Black edge indicates cross-linked site of Snap25.
Human Hippocampal Progenitor/Stem Cell Line Hpc0a07/03c, supplied by Reneuron Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+brain+vascular+pericytes+%28hpcs/pm37849234-57-12-19?v=Reneuron+Inc
Average 90 stars, based on 1 article reviews
human hippocampal progenitor/stem cell line hpc0a07/03c - by Bioz Stars, 2026-07
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anti rnase l  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti rnase l
( A ) Cross-link maps of selected SNARE proteins (dataset 1). Protein domains are depicted based on UniProt database and previous publications [*, from ]. Identified cross-links located in unique and shared peptides of <t>Stx1a</t> and Stx1b are represented as solid and dotted lines, respectively. ( B ) Results of Stxbp1 and control peptide array experiments. Examples of specific, nonspecific, and no binding signals are depicted. Arrowheads indicate antibody positive controls. Quantification and peptide sequences (two independent and two technical replicates) are described in fig. S9B and table S4. ( C ) Comparison of binding regions determined by peptide array (boxes colored in red) and XL-MS (edges). The number of samples in which the cross-links were identified is indicated on the edges (dataset 1). For peptide array assays, full-length proteins are shown as circles, and the protein used to generate peptide sequences in the arrays (Stx1b) is represented as sequence bars. Yellow edges match arrows with the same color in (D). ( D ) Cross-link mapping of Stxbp1 interactions. Stxbp1 (green) and Stx1 (blue) cross-linked sites were mapped onto the Stxbp1-Stx1 complex structure (PDB 3C98; Stx1 in closed conformation). Cross-links are indicated in red (if below DSSO maximal distance restraint) or in magenta (if exceeding DSSO maximal distance restraint). Yellow sticks show the two lysine residues of Stx1 (Lys45 and Lys55) found outside of the binding region with Stxbp1 as defined by the peptide array. Black edge indicates cross-linked site of Snap25.
Anti Rnase L, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+brain+vascular+pericytes+%28hpcs/pmc06609161-442-9-11?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
anti rnase l - by Bioz Stars, 2026-07
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goat anti syntaxin 2 antibody  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology goat anti syntaxin 2 antibody
( A ) Cross-link maps of selected SNARE proteins (dataset 1). Protein domains are depicted based on UniProt database and previous publications [*, from ]. Identified cross-links located in unique and shared peptides of <t>Stx1a</t> and Stx1b are represented as solid and dotted lines, respectively. ( B ) Results of Stxbp1 and control peptide array experiments. Examples of specific, nonspecific, and no binding signals are depicted. Arrowheads indicate antibody positive controls. Quantification and peptide sequences (two independent and two technical replicates) are described in fig. S9B and table S4. ( C ) Comparison of binding regions determined by peptide array (boxes colored in red) and XL-MS (edges). The number of samples in which the cross-links were identified is indicated on the edges (dataset 1). For peptide array assays, full-length proteins are shown as circles, and the protein used to generate peptide sequences in the arrays (Stx1b) is represented as sequence bars. Yellow edges match arrows with the same color in (D). ( D ) Cross-link mapping of Stxbp1 interactions. Stxbp1 (green) and Stx1 (blue) cross-linked sites were mapped onto the Stxbp1-Stx1 complex structure (PDB 3C98; Stx1 in closed conformation). Cross-links are indicated in red (if below DSSO maximal distance restraint) or in magenta (if exceeding DSSO maximal distance restraint). Yellow sticks show the two lysine residues of Stx1 (Lys45 and Lys55) found outside of the binding region with Stxbp1 as defined by the peptide array. Black edge indicates cross-linked site of Snap25.
Goat Anti Syntaxin 2 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
goat anti syntaxin 2 antibody - by Bioz Stars, 2026-07
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drosophila syntaxin  (Developmental Studies Hybridoma Bank)
93
Developmental Studies Hybridoma Bank drosophila syntaxin
( a , b ) Plot of P -values indicating significance of compound rescue ( a ) and summary of compound response ( b ) of ras G12V and ras G12V p53 Ri pten Ri apc Ri animals. P -values ( a ) were obtained by comparing the dissemination phenotype after compound feeding to dimethyl sulfoxide (DMSO) fed flies (dissemination plots can be found in ). Blue dots represent statistically significant results. ( c ) Quantification of dissemination in ras G12V pten Ri and ras G12V p53 Ri apc Ri animals treated with BEZ235. ( d ) Western blot analysis of PI3K pathway output from hindguts with indicated genotypes 7 days after induction of transgenes and quantification. Syn, <t>Syntaxin</t> (loading control). ( e ) Time-course analysis of PI3K pathway activation status in control and ras G12V p53 Ri pten Ri apc Ri hindguts. ( f ) Western blot analysis of the biochemical response by ras G12V and ras G12V p53 Ri pten Ri apc Ri animals to PI3K pathway inhibitors. ( g ) Quantification of dissemination in indicated genotypes treated with BEZ235 or DMSO. ( h ) Schematic illustration of the mechanism of resistance to BEZ235: genetically activating mTORC1 promotes BEZ235 sensitivity. ( d , e ) Each data point represents the average response of two to five biological replicates with ten hindguts per replicate; error bars: s.e.m. ( a – c , g ) n =2 replicates, 30 flies per replicate; error bars: s.e.m. * P <0.01 and ** P <0.05 (Fisher's exact test). Compound doses reflect concentrations in the food. Uncropped gels with molecular markers for d and f can be found in .
Drosophila Syntaxin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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drosophila syntaxin - by Bioz Stars, 2026-07
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anti hpcal1  (Proteintech)
93
Proteintech anti hpcal1
( a , b ) Plot of P -values indicating significance of compound rescue ( a ) and summary of compound response ( b ) of ras G12V and ras G12V p53 Ri pten Ri apc Ri animals. P -values ( a ) were obtained by comparing the dissemination phenotype after compound feeding to dimethyl sulfoxide (DMSO) fed flies (dissemination plots can be found in ). Blue dots represent statistically significant results. ( c ) Quantification of dissemination in ras G12V pten Ri and ras G12V p53 Ri apc Ri animals treated with BEZ235. ( d ) Western blot analysis of PI3K pathway output from hindguts with indicated genotypes 7 days after induction of transgenes and quantification. Syn, <t>Syntaxin</t> (loading control). ( e ) Time-course analysis of PI3K pathway activation status in control and ras G12V p53 Ri pten Ri apc Ri hindguts. ( f ) Western blot analysis of the biochemical response by ras G12V and ras G12V p53 Ri pten Ri apc Ri animals to PI3K pathway inhibitors. ( g ) Quantification of dissemination in indicated genotypes treated with BEZ235 or DMSO. ( h ) Schematic illustration of the mechanism of resistance to BEZ235: genetically activating mTORC1 promotes BEZ235 sensitivity. ( d , e ) Each data point represents the average response of two to five biological replicates with ten hindguts per replicate; error bars: s.e.m. ( a – c , g ) n =2 replicates, 30 flies per replicate; error bars: s.e.m. * P <0.01 and ** P <0.05 (Fisher's exact test). Compound doses reflect concentrations in the food. Uncropped gels with molecular markers for d and f can be found in .
Anti Hpcal1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+brain+vascular+pericytes+%28hpcs/pmc11800645-35-3-6?v=Proteintech
Average 93 stars, based on 1 article reviews
anti hpcal1 - by Bioz Stars, 2026-07
93/100 stars
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Image Search Results


( A ) Cross-link maps of selected SNARE proteins (dataset 1). Protein domains are depicted based on UniProt database and previous publications [*, from ]. Identified cross-links located in unique and shared peptides of Stx1a and Stx1b are represented as solid and dotted lines, respectively. ( B ) Results of Stxbp1 and control peptide array experiments. Examples of specific, nonspecific, and no binding signals are depicted. Arrowheads indicate antibody positive controls. Quantification and peptide sequences (two independent and two technical replicates) are described in fig. S9B and table S4. ( C ) Comparison of binding regions determined by peptide array (boxes colored in red) and XL-MS (edges). The number of samples in which the cross-links were identified is indicated on the edges (dataset 1). For peptide array assays, full-length proteins are shown as circles, and the protein used to generate peptide sequences in the arrays (Stx1b) is represented as sequence bars. Yellow edges match arrows with the same color in (D). ( D ) Cross-link mapping of Stxbp1 interactions. Stxbp1 (green) and Stx1 (blue) cross-linked sites were mapped onto the Stxbp1-Stx1 complex structure (PDB 3C98; Stx1 in closed conformation). Cross-links are indicated in red (if below DSSO maximal distance restraint) or in magenta (if exceeding DSSO maximal distance restraint). Yellow sticks show the two lysine residues of Stx1 (Lys45 and Lys55) found outside of the binding region with Stxbp1 as defined by the peptide array. Black edge indicates cross-linked site of Snap25.

Journal: Science Advances

Article Title: Stitching the synapse: Cross-linking mass spectrometry into resolving synaptic protein interactions

doi: 10.1126/sciadv.aax5783

Figure Lengend Snippet: ( A ) Cross-link maps of selected SNARE proteins (dataset 1). Protein domains are depicted based on UniProt database and previous publications [*, from ]. Identified cross-links located in unique and shared peptides of Stx1a and Stx1b are represented as solid and dotted lines, respectively. ( B ) Results of Stxbp1 and control peptide array experiments. Examples of specific, nonspecific, and no binding signals are depicted. Arrowheads indicate antibody positive controls. Quantification and peptide sequences (two independent and two technical replicates) are described in fig. S9B and table S4. ( C ) Comparison of binding regions determined by peptide array (boxes colored in red) and XL-MS (edges). The number of samples in which the cross-links were identified is indicated on the edges (dataset 1). For peptide array assays, full-length proteins are shown as circles, and the protein used to generate peptide sequences in the arrays (Stx1b) is represented as sequence bars. Yellow edges match arrows with the same color in (D). ( D ) Cross-link mapping of Stxbp1 interactions. Stxbp1 (green) and Stx1 (blue) cross-linked sites were mapped onto the Stxbp1-Stx1 complex structure (PDB 3C98; Stx1 in closed conformation). Cross-links are indicated in red (if below DSSO maximal distance restraint) or in magenta (if exceeding DSSO maximal distance restraint). Yellow sticks show the two lysine residues of Stx1 (Lys45 and Lys55) found outside of the binding region with Stxbp1 as defined by the peptide array. Black edge indicates cross-linked site of Snap25.

Article Snippet: Mouse Stx1a (NM_016801) was cloned into the pCMV6-Entry vector, yielding Stx1a-pCMV6-Entry including a C-terminal Myc-Flag tag (OriGene MR203927).

Techniques: Peptide Microarray, Binding Assay, Sequencing

( a , b ) Plot of P -values indicating significance of compound rescue ( a ) and summary of compound response ( b ) of ras G12V and ras G12V p53 Ri pten Ri apc Ri animals. P -values ( a ) were obtained by comparing the dissemination phenotype after compound feeding to dimethyl sulfoxide (DMSO) fed flies (dissemination plots can be found in ). Blue dots represent statistically significant results. ( c ) Quantification of dissemination in ras G12V pten Ri and ras G12V p53 Ri apc Ri animals treated with BEZ235. ( d ) Western blot analysis of PI3K pathway output from hindguts with indicated genotypes 7 days after induction of transgenes and quantification. Syn, Syntaxin (loading control). ( e ) Time-course analysis of PI3K pathway activation status in control and ras G12V p53 Ri pten Ri apc Ri hindguts. ( f ) Western blot analysis of the biochemical response by ras G12V and ras G12V p53 Ri pten Ri apc Ri animals to PI3K pathway inhibitors. ( g ) Quantification of dissemination in indicated genotypes treated with BEZ235 or DMSO. ( h ) Schematic illustration of the mechanism of resistance to BEZ235: genetically activating mTORC1 promotes BEZ235 sensitivity. ( d , e ) Each data point represents the average response of two to five biological replicates with ten hindguts per replicate; error bars: s.e.m. ( a – c , g ) n =2 replicates, 30 flies per replicate; error bars: s.e.m. * P <0.01 and ** P <0.05 (Fisher's exact test). Compound doses reflect concentrations in the food. Uncropped gels with molecular markers for d and f can be found in .

Journal: Nature Communications

Article Title: Functional exploration of colorectal cancer genomes using Drosophila

doi: 10.1038/ncomms13615

Figure Lengend Snippet: ( a , b ) Plot of P -values indicating significance of compound rescue ( a ) and summary of compound response ( b ) of ras G12V and ras G12V p53 Ri pten Ri apc Ri animals. P -values ( a ) were obtained by comparing the dissemination phenotype after compound feeding to dimethyl sulfoxide (DMSO) fed flies (dissemination plots can be found in ). Blue dots represent statistically significant results. ( c ) Quantification of dissemination in ras G12V pten Ri and ras G12V p53 Ri apc Ri animals treated with BEZ235. ( d ) Western blot analysis of PI3K pathway output from hindguts with indicated genotypes 7 days after induction of transgenes and quantification. Syn, Syntaxin (loading control). ( e ) Time-course analysis of PI3K pathway activation status in control and ras G12V p53 Ri pten Ri apc Ri hindguts. ( f ) Western blot analysis of the biochemical response by ras G12V and ras G12V p53 Ri pten Ri apc Ri animals to PI3K pathway inhibitors. ( g ) Quantification of dissemination in indicated genotypes treated with BEZ235 or DMSO. ( h ) Schematic illustration of the mechanism of resistance to BEZ235: genetically activating mTORC1 promotes BEZ235 sensitivity. ( d , e ) Each data point represents the average response of two to five biological replicates with ten hindguts per replicate; error bars: s.e.m. ( a – c , g ) n =2 replicates, 30 flies per replicate; error bars: s.e.m. * P <0.01 and ** P <0.05 (Fisher's exact test). Compound doses reflect concentrations in the food. Uncropped gels with molecular markers for d and f can be found in .

Article Snippet: Primary antibodies were as follows: rabbit anti Drosophila phospho-AKT (p-Ser505; Drosophila equivalent of mammalian AKT p-Ser473, 1:1,000, Cell Signaling, catalogue number 4054S), rabbit anti-mouse AKT (1:1,000, Cell Signaling, catalogue number 4691S), rabbit anti-mouse phospho-4EBP (Thr37/46, 1:1,000, Cell Signaling, catalogue number 2855S), rabbit anti-human phospho-AKT (Ser473, 1:1,000, Cell Signaling, catalogue number 4060S), mouse anti-chicken α-actin (1:1,000, DSHB catalogue number JLA20-s), rabbit anti-GFP (1:2,000, Sigma, catalogue number G1544), rabbit anti Drosophila P53 (1:1,000, DSHB, catalogue number H3), rabbit anti Drosophila Pten (1:1,000, gift from A. Wodarz ), mouse anti-dpERK (Thr183/Tyr185, 1:2,000, Sigma, catalogue number M8159), rabbit anti human phospho-S6 (Ser 235/236, 1:1,000, Cell Signaling, catalogue number 2211) and mouse anti Drosophila Syntaxin (1:1,000, DSHB, catalogue number 8C3).

Techniques: Western Blot, Control, Activation Assay

( a ) Western blot analysis of PI3K signalling pathway output in ras G12V and ras G12V p53 Ri pten Ri apc Ri hindguts after 1 day feeding of SC79 at indicated doses. Syn, Syntaxin (loading control);ten hindguts per replicate. ( b ) Quantification of dissemination in ras G12V and ras G12V p53 Ri pten Ri apc Ri animals after sequential treatment with BEZ235 and indicated doses of SC79. ( c ) Quantification of dissemination in ras G12V and ras G12V p53 Ri pten Ri apc Ri animals after two different treatment schedules of SC79/BEZ235 and each drug alone. ( b , c ) n =2 replicates, 30 flies per replicate; error bars: s.e.m. * P <0.01 and ** P <0.05 (Fisher's exact test). ( b ) *Variable response; not all replicates show significant rescue. Drug doses reflect concentrations in the food. Uncropped gels used to generate panel a can be found in .

Journal: Nature Communications

Article Title: Functional exploration of colorectal cancer genomes using Drosophila

doi: 10.1038/ncomms13615

Figure Lengend Snippet: ( a ) Western blot analysis of PI3K signalling pathway output in ras G12V and ras G12V p53 Ri pten Ri apc Ri hindguts after 1 day feeding of SC79 at indicated doses. Syn, Syntaxin (loading control);ten hindguts per replicate. ( b ) Quantification of dissemination in ras G12V and ras G12V p53 Ri pten Ri apc Ri animals after sequential treatment with BEZ235 and indicated doses of SC79. ( c ) Quantification of dissemination in ras G12V and ras G12V p53 Ri pten Ri apc Ri animals after two different treatment schedules of SC79/BEZ235 and each drug alone. ( b , c ) n =2 replicates, 30 flies per replicate; error bars: s.e.m. * P <0.01 and ** P <0.05 (Fisher's exact test). ( b ) *Variable response; not all replicates show significant rescue. Drug doses reflect concentrations in the food. Uncropped gels used to generate panel a can be found in .

Article Snippet: Primary antibodies were as follows: rabbit anti Drosophila phospho-AKT (p-Ser505; Drosophila equivalent of mammalian AKT p-Ser473, 1:1,000, Cell Signaling, catalogue number 4054S), rabbit anti-mouse AKT (1:1,000, Cell Signaling, catalogue number 4691S), rabbit anti-mouse phospho-4EBP (Thr37/46, 1:1,000, Cell Signaling, catalogue number 2855S), rabbit anti-human phospho-AKT (Ser473, 1:1,000, Cell Signaling, catalogue number 4060S), mouse anti-chicken α-actin (1:1,000, DSHB catalogue number JLA20-s), rabbit anti-GFP (1:2,000, Sigma, catalogue number G1544), rabbit anti Drosophila P53 (1:1,000, DSHB, catalogue number H3), rabbit anti Drosophila Pten (1:1,000, gift from A. Wodarz ), mouse anti-dpERK (Thr183/Tyr185, 1:2,000, Sigma, catalogue number M8159), rabbit anti human phospho-S6 (Ser 235/236, 1:1,000, Cell Signaling, catalogue number 2211) and mouse anti Drosophila Syntaxin (1:1,000, DSHB, catalogue number 8C3).

Techniques: Western Blot, Control